铜萃取剂BK992和LIX984的性能研究

研究羟肟类铜萃取剂的结构与性能的关系。试验研究了铜萃取剂BK992和LIX984的对比萃取及混合萃取性能指标 ,试验表明BK992与LIX984的使用性能相当 ,两种铜萃取剂相互混合不影响使用性能指标。     

真空热处理工艺参数的选取

通过多种材料、多种工件、多批次的加工，对常见真空热处理工艺参数的确定，摸索出一些经验公式，并在实际生产中取得很好效果。     

心脏黏液瘤的超微结构研究

对8例心脏黏液瘤进行了临床病理总结，着重进行了超微结构的研究，结果表明黏液瘤细胞有4种超微结构表现：（1）原始间叶细胞；（2）纤维母细胞样；（3）肌纤维母细胞样；（4）上皮样。提出心脏黏液瘤细胞起源于原始间叶细胞，有纤维母细胞、肌及上皮样分化。并且提出瘤细胞参与黏液瘤基质的形成。     

等离子体技术及其在“三废”处理中的应用

阐述等离子体的概念、分类、特点和作用机理,从大气污染、水污染、固体废物治理三个方面简述在"三废"处理中各种等离子体技术的应用及特点,分析其存在的问题,展示了等离子体技术在环境保护中的应用前景.     

河口区积极探索水利工程对生态环境影响的对策

东营市河口区坚持科学发展观,努力探索水利工程与生态环境协调发展的路子,认真编制流域综合规划,把生态环境保护融入到水利建设的各项环节之中。通过建立科学的技术支撑体系,实行公众参与,加强社会舆论监督;加强河道保护和管理,维护良好的河道治理开发秩序;加强水资源统一管理,建设节水防污型社会,在加强生态建设、改善生态环境等方面,取得了一定的成绩。     

改性壳聚糖的研究进展

壳聚糖是甲壳素脱乙酰化的天然聚合物,有着良好的生物学特性.综述了化学改性壳聚糖的方法和产物特性,及改性壳聚糖在医药、工业废水处理和食品工业等领域的应用.     

二次型时频分析技术的开发与应用

基于二次型的时频分析是高分辨率时频分析技术的有效方法之一，该项技术能够有效反映时变信号的频谱特性，具有分辨率高、能量集中和跟踪瞬时频率的特点。从时变信号的特点出发，阐述了二次型时频分析技术的基本方法原理，采用正演模型分析验证了该方法的有效性，并在实际地震资料中应用于识别物源方向、划分沉积相带、预测储层结构以及相对厚度等诸多方面，均取得了较好的效果。     

健康长江评价指标体系与标准研究

科学的指标体系和标准是评价河流健康状况的技术依据。根据科学性、代表性、创新性等原则，运用主成分分析法和聚类分析法．构建了健康长江评价指标体系，包括河岸稳定性、河床稳定性等19个定量指标和水系连通性、河岸栖息地状况和珍稀水生动物存活状况3个定性指标；给出了相应指标的量化评价方法，并通过国内外相关研究成果的综合分析．提出了健康长江的评价标准及分类标准，为维持河流健康理论发展提供了新方法。     

局部施肥对植株生长及根系形态的影响

本文论述了局部施肥对植株地上部与地下部的影响。局部施肥促进了施肥区根系的发达 ,第一侧根与第二侧根较多 ,根的吸收表面积增多 ,根鲜重增加 ,对养分的吸收能力增强 ,但整个根系与地上部的干鲜重下降。     

Activation of PKCalpha downstream from caspases during apoptosis induced by 7-hydroxystaurosporine or the topoisomerase inhibitors, camptothecin and etoposide, in human myeloid leukemia HL60 cells

We previously demonstrated that the anticancer agent and protein kinase C (PKC) inhibitor 7-hydroxystaurosporine (UCN-01) induces apoptosis independently of p53 and protein synthesis in HL60 cells. We now report the associated changes of PKC isoforms. PKCalpha, betaI, betaII, delta, and zeta activities were measured after immunoprecipitation of cytosols from UCN-01-treated HL60 cells. UCN-01 had no effect on PKCzeta and inhibited kinase activity of PKCbetaI, betaII, and delta. PKCalpha activity was initially inhibited at 1 h, and subsequently increased as cells underwent apoptosis 3 h after the beginning of UCN-01 treatment. Camptothecin (CPT) and etoposide (VP-16) also markedly enhanced PKCalpha activity during apoptosis in HL60 cells. However, CPT did not affect PKCbetaI, betaII and zeta, and activated PKCdelta. PKCalpha activation was not due to increased protein levels or proteolytic cleavage but was associated with PKCalpha autophosphorylation in vitro and increased phosphorylation in vivo. We also found that not only PKC delta but also PKC betaI was proteolytically activated in HL60 cells during apoptosis. The PKCalpha activation and hyperphosphorylation were abrogated by N-benzyloxycarbonyl-Val-Ala-Asp(O-methyl)-fluoromethylketone (z-VAD-fmk) under conditions that abrogated apoptosis. z-VAD-fmk also prevented PKCdelta and betaI proteolytic activation. Together these findings suggest that caspases regulate PKC activity during apoptosis in HL60 cells. At least two modes of activation were observed: hyperphosphorylation for PKCalpha and proteolytic activation for PKC delta and betaI.